diff -Nru rna-star-2.5.4a+dfsg/CHANGES.md rna-star-2.5.4b+dfsg/CHANGES.md --- rna-star-2.5.4a+dfsg/CHANGES.md 2018-01-25 15:51:24.000000000 +0000 +++ rna-star-2.5.4b+dfsg/CHANGES.md 2018-02-09 16:00:32.000000000 +0000 @@ -1,9 +1,16 @@ +STAR 2.5.4b 2018/02/09 +====================== + +* Recompiled Linux executables with the correct version tag. +* Updated manual. + STAR 2.5.4a 2018/01/23 +====================== ### New features: * Implemented read group ID output as the last column of the Chimeric.out.junction file. * Implemented --readFilesPrefix option for specifying prefix (e.g. directory path) for the file names in --readFilesIn . -* Implemented stanard SAM attrbiute "MC" to output the mate's CIGAR. Add MC to the list of attributes in the --outSAMattribute option. +* Implemented standard SAM attribute "MC" to output the mate's CIGAR. Add MC to the list of attributes in the --outSAMattribute option. * Implemented the ability to input the reads from unmapped SAM/BAM file: --readFilesType SAM SE[PE] for single-end [paired-end] reads to read from the SAM file specified, as usual in --readFilesIn. For BAM files, in addition, specify --readFilesCommand samtools view -h . * Implemented --seedSplitMin option which was previously hardcoded at 12. his will allow mapping of mates shorter than 12nt. * Implemented --outFilterIntronStrands None option to switch off filtering by strand consistency of junctions. @@ -11,9 +18,9 @@ ### Bug fixes: * Fixed a bug in chimeric detection code which sometimes led to uninitialized memory access. The chimeric output may change for a very small number of reads. -* Fixed a problem with --alignEndsProtrude implementation which prevented output of alignments with protruded ends. +* Fixed a problem with --alignEndsProtrude implementation which prevented the output of alignments with protruded ends. * Fixed a bug which set non-primary bit 0x100 in the SAM FLAG for unmapped mates. -* Fixed a bug in liftOver command that output an extra field in the GTF file. +* Fixed a bug in liftOver command that output an extra field in the GTF file. * Fixed a problem that can arise for very small genomes while using --alignIntronMax 1. STAR 2.5.3a 2017/03/17 diff -Nru rna-star-2.5.4a+dfsg/debian/changelog rna-star-2.5.4b+dfsg/debian/changelog --- rna-star-2.5.4a+dfsg/debian/changelog 2018-01-26 10:32:21.000000000 +0000 +++ rna-star-2.5.4b+dfsg/debian/changelog 2018-02-11 12:37:19.000000000 +0000 @@ -1,3 +1,10 @@ +rna-star (2.5.4b+dfsg-1) unstable; urgency=medium + + * New upstream release. + * Respect nocheck flag in test override. + + -- Sascha Steinbiss Sun, 11 Feb 2018 13:37:19 +0100 + rna-star (2.5.4a+dfsg-1) unstable; urgency=medium [ Sascha Steinbiss ] diff -Nru rna-star-2.5.4a+dfsg/debian/rules rna-star-2.5.4b+dfsg/debian/rules --- rna-star-2.5.4a+dfsg/debian/rules 2018-01-26 10:32:21.000000000 +0000 +++ rna-star-2.5.4b+dfsg/debian/rules 2018-02-11 12:37:19.000000000 +0000 @@ -25,4 +25,6 @@ dh_compress --exclude=.pdf override_dh_auto_test: +ifeq (,$(filter nocheck,$(DEB_BUILD_OPTIONS))) debian/tests/run_test.sh $(CURDIR)/source/STAR +endif diff -Nru rna-star-2.5.4a+dfsg/doc/STARmanual.pdf 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+0000250735 00000 n +0000250863 00000 n trailer -<< /Size 850 -/Root 848 0 R -/Info 849 0 R -/ID [ ] >> +<< /Size 859 +/Root 857 0 R +/Info 858 0 R +/ID [ ] >> startxref -249028 +251138 %%EOF diff -Nru rna-star-2.5.4a+dfsg/extras/doc-latex/parametersDefault.tex rna-star-2.5.4b+dfsg/extras/doc-latex/parametersDefault.tex --- rna-star-2.5.4a+dfsg/extras/doc-latex/parametersDefault.tex 2018-01-25 15:51:24.000000000 +0000 +++ rna-star-2.5.4b+dfsg/extras/doc-latex/parametersDefault.tex 2018-02-09 16:00:32.000000000 +0000 @@ -63,6 +63,9 @@ \optName{genomeFileSizes} \optValue{0} \optLine{uint(s){\textgreater}0: genome files exact sizes in bytes. Typically, this should not be defined by the user.} +\optName{genomeConsensusFile} + \optValue{-} + \optLine{string: VCF file with consensus SNPs (i.e. alternative allele is the major (AF{\textgreater}0.5) allele)} \end{optTable} \optSection{Genome Indexing Parameters - only used with --runMode genomeGenerate}\label{Genome_Indexing_Parameters_-_only_used_with_--runMode_genomeGenerate} \begin{optTable} @@ -121,9 +124,21 @@ \end{optTable} \optSection{Read Parameters}\label{Read_Parameters} \begin{optTable} +\optName{readFilesType} + \optValue{Fastx} + \optLine{string: format of input read files} +\begin{optOptTable} + \optOpt{Fastx} \optOptLine{FASTA or FASTQ} + \optOpt{SAM SE} \optOptLine{SAM or BAM single-end reads; for BAM use --readFilesCommand samtools view} + \optOpt{SAM PE} \optOptLine{SAM or BAM paired-end reads; for BAM use --readFilesCommand samtools view} +\end{optOptTable} \optName{readFilesIn} \optValue{Read1 Read2} \optLine{string(s): paths to files that contain input read1 (and, if needed, read2)} +\optName{readFilesPrefix} + \optValue{-} + \optLine{string: preifx for the read files names, i.e. it will be added in front of the strings in --readFilesIn} + \optLine{-: no prefix} \optName{readFilesCommand} \optValue{-} \optLine{string(s): command line to execute for each of the input file. This command should generate FASTA or FASTQ text and send it to stdout} diff -Nru rna-star-2.5.4a+dfsg/extras/doc-latex/STARmanual.tex rna-star-2.5.4b+dfsg/extras/doc-latex/STARmanual.tex --- rna-star-2.5.4a+dfsg/extras/doc-latex/STARmanual.tex 2018-01-25 15:51:24.000000000 +0000 +++ rna-star-2.5.4b+dfsg/extras/doc-latex/STARmanual.tex 2018-02-09 16:00:32.000000000 +0000 @@ -34,7 +34,7 @@ \newcommand{\sechyperref}[1]{\hyperref[#1]{Section \ref{#1}. \nameref{#1}}} -\title{STAR manual 2.5.3a} +\title{STAR manual 2.5.4b} \author{Alexander Dobin\\ dobin@cshl.edu} \maketitle @@ -112,7 +112,7 @@ \opt{genomeDir} specifies path to the directory (henceforth called "genome directory" where the genome indices are stored. This directory has to be created (with \code{mkdir}) before STAR run and needs to have writing permissions. The file system needs to have at least 100GB of disk space available for a typical mammalian genome. It is recommended to remove all files from the genome directory before running the genome generation step. This directory path will have to be supplied at the mapping step to identify the reference genome. \item[] -\opt{genomeFastaFiles} specified one or more FASTA files with the genome reference sequences. Multiple reference sequences (henceforth called “chromosomes”) are allowed for each fasta file. You can rename the chromosomes’ names in the chrName.txt keeping the order of the chromosomes in the file: the names from this file will be used in all output alignment files (such as .sam). The tabs are not allowed in chromosomes’ names, and spaces are not recommended. +\opt{genomeFastaFiles} specifies one or more FASTA files with the genome reference sequences. Multiple reference sequences (henceforth called “chromosomes”) are allowed for each fasta file. You can rename the chromosomes’ names in the chrName.txt keeping the order of the chromosomes in the file: the names from this file will be used in all output alignment files (such as .sam). The tabs are not allowed in chromosomes’ names, and spaces are not recommended. \item[] \opt{sjdbGTFfile} specifies the path to the file with annotated transcripts in the standard GTF format. STAR will extract splice junctions from this file and use them to greatly improve accuracy of the mapping. While this is optional, and STAR can be run without annotations, using annotations is \textbf{highly recommended} whenever they are available. Starting from 2.4.1a, the annotations can also be included on the fly at the mapping step. @@ -170,12 +170,18 @@ \opt{genomeDir} specifies path to the genome directory where genome indices where generated (see \sechyperref{Generating_genome_indexes}). \item[] -\opt{readFilesIn} name(s) (with path) of the files containing the sequences to be mapped (e.g. RNA-seq FASTQ files). If using Illumina paired-end reads, the \optvr{read1} and \optv{read2} files have to be supplied. STAR can process both FASTA and FASTQ files. Multi-line (i.e. sequence split in multiple lines) FASTA file are supported. If the read files are compressed, use the \opt{readFilesCommand} \optvr{UncompressionCommand} option, where \optvr{UncompressionCommand} is the un-compression command that takes the file name as input parameter, and sends the uncompressed output to stdout. For example, for gzipped files (*.gz) use +\opt{readFilesIn} name(s) (with path) of the files containing the sequences to be mapped (e.g. RNA-seq FASTQ files). If using Illumina paired-end reads, the \optvr{read1} and \optvr{read2} files have to be supplied. STAR can process both FASTA and FASTQ files. Multi-line (i.e. sequence split in multiple lines) FASTA (but not FASTQ) files are supported. + +If the read files are compressed, use the \opt{readFilesCommand} \optvr{UncompressionCommand} option, where \optvr{UncompressionCommand} is the un-compression command that takes the file name as input parameter, and sends the uncompressed output to stdout. For example, for gzipped files (*.gz) use \code{\opt{readFilesCommand} \optv{zcat}} OR \code{\opt{readFilesCommand} \optv{gunzip -c}}. For bzip2-compressed files, use \code{\opt{readFilesCommand} \optv{bunzip2 -c}}. + +Multiple samples can be mapped in one job. For single-end reads use a comma separated list (no spaces around commas), e.g. +\opt{readFilesIn} \optv{sample1.fq,sample2.fq,sample3.fq}. For paired-end reads, use comma separated list for read1 /space/ comma separated list for read2, e.g.: \opt{readFilesIn} \optv{sample1read1.fq,sample2read1.fq,sample3read1.fq sample1read2.fq,sample2read2.fq,sample3read2.fq}. + \end{itemize} \subsection{Advanced options.} @@ -203,8 +209,8 @@ \opt{outFilterMismatchNmax} 999\\ maximum number of mismatches per pair, large number switches off this filter \item[] -%\opt{outFilterMismatchNoverReadLmax} 0.04\\ -max number of mismatches per pair relative to read length: for 2x100b, max number of mismatches is 0.06*200=8 for the paired read +\opt{outFilterMismatchNoverReadLmax} 0.04\\ +max number of mismatches per pair relative to read length: for 2x100b, max number of mismatches is 0.04*200=8 for the paired read \item[] \opt{alignIntronMin} 20\\ minimum intron length diff -Nru rna-star-2.5.4a+dfsg/source/VERSION rna-star-2.5.4b+dfsg/source/VERSION --- rna-star-2.5.4a+dfsg/source/VERSION 2018-01-25 15:51:24.000000000 +0000 +++ rna-star-2.5.4b+dfsg/source/VERSION 2018-02-09 16:00:32.000000000 +0000 @@ -1 +1 @@ -#define STAR_VERSION "STAR_2.5.4a" +#define STAR_VERSION "STAR_2.5.4b"