Binary package “primer3” in ubuntu disco

tool to design flanking oligo nucleotides for DNA amplification

 Primer3 picks primers for Polymerase Chain Reactions (PCRs), considering as
 criteria oligonucleotide melting temperature, size, GC content and
 primer-dimer possibilities, PCR product size, positional constraints within
 the source sequence, and miscellaneous other constraints. All of these
 criteria are user-specifiable as constraints, and some are specifiable as
 terms in an objective function that characterizes an optimal primer pair.