estimate PCR barcode primers quality

 DNA barcoding is a tool for characterizing the species origin using a
 short sequence from a standard position and agreed upon position in the
 genome. To be used as a DNA barcode, a genome locus should vary among
 individuals of the same species only to a minor degree and it should
 vary among species very quickly. From a practical point of view, a
 barcode locus should be flanked by two conserved regions to design PCR
 primers. Several manually discovered barcode loci like COI, rbcL, 18S,
 16S and 23S rDNA, or trnH-ps are routinely used today, but no objective
 function has been described to measure their quality in terms of
 universality (barcode coverage, Bc ) or in terms of taxonomical
 discrimination capacity (barcode specificity, Bs ).
 .
 ecoPCR is an electronic PCR software developed by LECA and
 Helix-Project. It helps to estimate Barcode primers quality. In
 conjunction with OBITools you can postprocess ecoPCR output to compute
 barcode coverage and barcode specificity. New barcode primers can be
 developed using the ecoPrimers software